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IgG (H+L) Secondary Antibody – Goat

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS128200
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Background: Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use… More details

$155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG (H+L), Secondary Antibody
Full Product Name
HRP Goat Anti-Rabbit IgG (H+L) Antibody
Product Synonym Names
HRP Goat Anti-Rabbit IgG (H+L): N/A
Isotype
Horseradish peroxidase conjugated IgG
Host
Goat
Species Reactivity
Rabbit
Form/Format
PBS with 0.75% BSA, 50% glycerol, pH 7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
Immunogen
Rabbit IgG
Conjugation
HRP
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for IgG (H+L) secondary antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry Parrafin (IHC-P), Dot Blot (DB)
Application Notes for IgG (H+L) secondary antibody
ELISA: 1:5000 - 1:10000
WB: 1:2000 - 1:10000
IHC: 1:50 - 1:200
IHC-P: 1:50 - 1:200

Western Blot (WB) of IgG (H+L) secondary antibody

Western blot analysis of extracts of HeLa cells,using GAPDH ( MBS127695 ) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody ( MBS128200 ) at 1:4000-1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s
Goat Anti-IgG (H+L) Secondary Antibody – Cat# MBS128200

Immunohistochemistry (IHC) of IgG (H+L) secondary antibody

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Goat Anti-IgG (H+L) Secondary Antibody, Immunohistochemistry (IHC) – Cat# MBS128200

Other Notes

Small volumes of IgG (H+L) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG (H+L) purchase

MBS128200 is a secondary antibody targeting IgG (H+L). Also known as HRP Goat Anti-Rabbit IgG (H+L) Antibody. Reactive in Rabbit. Host: Goat.
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